Results and conclusions will be reported on the following form:

(a hard copy will be provided - do not use this virtual version)

Genetics Project Report Form:

Name(s): ____________________________________________________

Semester: ___________________  Year:  __________________________

Project Title (e.g. monohybrid cross):  ____________________________

Mutant Studied (e.g. sepia eye color):  ____________________________

Results:

From reciprocal parental crosses:

a)  wild type virgin female  X  mutant male  >>>>  F₁(a) offspring:

1)  number of F₁(a) males (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

2)  number of F₁(a) females (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype  ________

(c)  mutant phenotype  ________

b)  mutant virgin female  X  wild type male  >>>>  F₁(b) offspring:

1)  number of F₁(b) males (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

2)  number of F₁(b) females (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

Results (continued):

From the F₁  X  F₁ crosses:

a)  F₁(a) female  X  F₁(a) male  >>>>  F₂(a) offspring:

1)  number of F₂(a) males (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

2)  number of F₂(a) females (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

b)  F₁(b) female  X  F₁(b) male  >>>>  F₂(b) offspring:

1)  number of F₂(b) males (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

2)  number of F₂(b) females (total for all vials counted):

(a)  normal (wild type) phenotype:  ________

(b)  intermediate phenotype:  ________

(c)  mutant phenotype:  ________

Data Analysis (Part I):

A test to determine if the total number of males and the total number of females counted in the entire study approximates a 1:1 ratio.

a)  Total # of males in the entire study  =  ________

b)  Total # of females in the entire study  =  ________

Perform a Chi Square Analysis on the totals:

Calculated Chi Square  =  ____________

Degrees of Freedom  =  ____________

Table Chi Square (Critical Value)  =  ____________

Conclusion:  ____________________________________________________________

Data Analysis (Part II):

A comparison of the expected results with the observed results from the reciprocal parental crosses.

a)  F₁(a) offspring:

1)  What is the expected phenotype for the F₁(a) males?

_______________________________________

Do the observed results fit the expected?

Yes  ________    No  ________

2)  What is the expected phenotype for the F₁(a) females?

_______________________________________

Do the observed results fit the expected?

Yes  ________    No  ________

b)  F₁(b) offspring:

1)  What is the expected phenotype for the F₁(b) males?

_______________________________________

Do the observed results fit the expected?

Yes  ________    No  ________

2)  What is the expected phenotype for the F₁(b) females?

_______________________________________

Do the observed results fit the expected?

Yes  ________    No  ________

Data Analysis (Part III):

A comparison of the expected results with the observed results from the F₁  X  F₁ crosses.

a)  F₂(a) offspring:

1)  What is the expected phenotypic ratio for the F₂(a) males?

_______________________________________

Does the observed ratio fit the expected ratio?

Yes  ________    No  ________

Calculated Chi Square (if appropriate):  ________

Table Chi Square (critical value):  ________

2)  What is the expected phenotypic ratio for the F₂(a) females?

_______________________________________

Does the observed ratio fit the expected ratio?

Yes  ________    No  ________

Calculated Chi Square (if appropriate):  ________

Table Chi Square (critical value):  ________

b)  F₂(b) offspring:

1)  What is the expected phenotypic ratio for the F₂(b) males?

_______________________________________

Does the observed ratio fit the expected ratio?

Yes  ________    No  ________

Calculated Chi Square (if appropriate):  ________

Table Chi Square (critical value):  ________

2)  What is the expected phenotypic ratio for the F₂(b) females?

_______________________________________

Does the observed ratio fit the expected ratio?

Yes  ________    No  ________

Calculated Chi Square (if appropriate):  ________

Table Chi Square (critical value):  ________

Commentary:

Comment upon any unusual observations or difficulties encountered in your study.

 

 

Conclusions:

Based on the analysis of the observed results:

a)  the mutant gene is an autosomal dominant:

Yes  ________    No  ________    Can't Tell  ________

b)  the mutant gene is an autosomal partial dominant:

Yes  ________    No  ________    Can't Tell  ________

c)  the mutant gene is an autosomal recessive:

Yes  ________    No  ________    Can't Tell  ________

d)  the mutant gene is an X- linked dominant:

Yes  ________    No  ________    Can't Tell  ________

e)  the mutant gene is an X- linked partial dominant:

Yes  ________    No  ________    Can't Tell  ________

f)  the mutant gene is an X- linked recessive:

Yes  ________    No  ________    Can't Tell  ________
 
 

CONTACT PERSON:  Robert Weibust - weibust@mnstate.edu

LAST UPDATE:  5/17/04

MSUM is an equal opportunity educator and employer