Research Protocols
Provost and Wallert Lab
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Lab Duties
General Interest
Gel/Antibody Protocols
Ethics and Science
Provost/Wallert Basic PPT Format
Protein Kinase Inhibitor/Activator Table
pH Meter Instructions
96 Well Plate Reader Blank Data Page
Preparing Posters
BCECF Tech Info
More Tech Info on BCECF
Lung Cancer Description
Fluromax Instructions
General Protocols from Chemicon
SDS-PAGE Gel Guide
Westernblotting / Transblotting Guide
Westernblotting Tips
Millipore's Westernblotting Book
(w/troubleshooting guide)
Upstate Biotech Westernblotting Book
(w/troubleshooting guide)
Introduction to Antibodies
(Chemicon website)
Stripping and Re-Probing Western Blots
Erasing X-Ray Film Protocol
Elisa Assay Protocol
Cell Culture Antibody Labeling Protocol
Assay Protocols
DNA Protocols
Phospholipase D (PLD) Assay Protocol
Protein Kinase C Translocation Protocol/Information
MAPK Activation Protocol/Information
Current Protocols in Cell Biology
- MAPK
GST Enzyme Assay
PEPCK, AST, Enolase, MDH... Enzyme Assay
Phosphate Assay (lipid) Protocol
Phospholipid Assay - M. Green Method
Endotoxin Assay
MMP9 Assay
MMP Activation Protocol
MMP9 Assay Background Handout
MTT Proliferation Assay Protocol
Sigma MTT product handout
96 well plate blank
Universal Protein Kinase Assay (Calbiochem)
Neonatal Heart Preparation
pHi (NHE) assay solution prep
Sample Prep for Kinases
(Pierce)
NHE null cell and Li poison protocol
Quick and Dirty Plasmid Prep Protocol
Qiagen DNA Purification Handbook
PCR General Protocol
Standard Agarose (DNA) gel electrophoresis protocol
Running a Fast DNA Gel Paper
Fast Agarose Gel Prep Prep Protocol
DNA Quantification Protocol
Background for DNA Quant
(good info)
Plasmid Stability Test
Plate Innoculation protocol
(for weak e.coli starters)
Subcloning point to consider
(promega)
Protein and Purification Protocols
Cell Assays and Culture Protocols
His Tag Purification Protocol
Dialyzing Samples Instructions
Ultrafiltration for concentrating protein samples protocol
Protein Assay Protocol (old version)
Protein Assay Protocol (better version)
Protein Assay for Cell Cultures (96 Well Plate Version)
Protein Expression and Purification Guide
Qiagen Protein Expression Handbook
Acetone Protein Precipitation Protocol
GFP Plate Reader Protocol
Heart Homogenization Protocol
Stress Fiber Protocol
Wound Assay Protocol
Bacterial Culture Protocol
Bacterial
Transformation Protocol
Protein Assay for Cell Cultures (96 Well Plate Version)
Transfection Protocol
Stable Transfection Cloning Protocol
Lysophosphatidic Acid (LPA) Preparation Protocol
Tissue / Cell Culture Protocol
Useful Cell Culture Numbers
Ponseau S Staining Protocol
Quick and Dirty Viable Cell Staining Protocol
Mammalian Cell Culture Feezing Protocol
Cell Culture Media Recipies
Counting Tissue Culture Cells w/ a Hemocytometer
Cleaning Incubator Protocol
Single Cell Cloning by Dilution Protocol(Corning)
PSN (NHE1 mutants) protocols
Soft Agar Colony Formation Assay Protocol
Tumor Paper Links
Look at these papers for ideas on how to do the xenoplant - metastasis type of experiements AND how the drugs were delivered. Pay attention to water sol. vs DMSO or other solvents.
An orthotopic model of lung cancer to analyze primary...
Antitumor Activity of ... Human Orthotopic Non Small Cell Lung Cancer Xenograft Models
Development of an orthotopic model to study the biology and therapy of primary human lung cancer in nude mice
Histology and sensitivity to anticancer drugs of two human non-small cell lung carcinomas implanted in the pleural cavity of nude mice
Hyaluronic Acid Paclitaxel Antitumor Efficacy against CD44 Human Ovarian Carcinoma Xenograft
Potentiation of Platinum Antitumor Effects in Human Lung Tumor