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BCBT 475 Research and
Communications I
BCBT 477 Proteomics & Adv Protein Expression BCBT 478 Advanced RNA Techniques Biochemistry and Biotechnology has been modified from two 5-credit courses, to five 2-credit courses. Each Biochemistry and Biotechnology Major must take Research and Communications I and II and three two credit techniques (proteomics, RNA...) courses to graduate. These changes are to help clarify grading and expectations. These changes will clarify the content and extent of your training on your transcript to your future employers or professional school. Biochemistry and Biotechnology Research and Communications I - students will work in teams to identify a faculty research mentor, prepare and present formal research proposals, and begin their research projects. This is a DragonCore writing intensive course. Proteomics and RNA Techniques - Students will learn cutting edge techniques and instruments while working in a research setting. The grade for these two courses are separate from Research and Communications I. |
| BBT 475 - Research and Communications I
(BCBT 475 Syllabus ) |
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| Research Project Description Project Form Master Sched Notebook Requirements | |||||
| Student
Expectatons - Be on time and attend every session - Each student is expected to work within her or his group as an fair share member. - Each student is expected to be respectful and responsible in interacting with their faculty mentor and with their peers. - Each student is expected to take the initiative in learning theory, topics and techniques in both the research and teaching blocks. - Each student is expected to clean up after themselves and be a good laboratory citizen. |
Time Expectations - In
general, each student should plan on spending 2 hrs of out
of class time for each hour of class time. This is a typical expectation by the University. As a result, each student should anticipate investing 15 – 20 hrs each week outside of class. This time includes homework, writing presentations, reading papers laboratory work fr your teaching blocks and laboratory time to work on your research. We will meet in the classroom each Friday before working with your research team. |
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Writing
and Research Assignments
Writing
Assignment I Due Aug 29Writing Assignment II Due Sept 12 Writing Assignment III Due Oct 3 Writing Assignment IV Due Oct 17 Research Assignment I Due Sept 5 Research Assignment II Due Oct 26 Research Assignment III Due Nov 7 NOTICE - It is your responsibility to turn in the completed assignments - No reminder will be given - Turn the assignments to the Chem/Biol Office - HA 407 by 4:00 Do NOT give to a professor or turn into a mailbox or under a door |
Research proposal
presentation handout Presentation guide handout Peer Team Member Evaluation Form - To be filled out at the end of the semster (Turn in with research notebook) Proposal Expectations -Required Reading: At the Bench. pp. 101-126 Undergraduate Research in the Molecular Science (URMS) an ASBMB & ACS sponsored Meeting Oct 17-18 2008 - Students will present their project plans at this meeting. - Make arraingments to be present Friday afternoon and Saturday morning - 1:00 pm. Attendance is required. |
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| BCBT 477 Proteomics & Advanced Protein Expression (Syllabus) | |||||
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8 - Oct 15 ; Drs. Marry & Provost There are several sections of this course. First we will be discussing protemics followed by advanced chromatography, protein expression recombinant protein expression and purification, as well as chromatography techniques including automated chromatography instruments. The laboratories for this section will be to determine the best expression conditions for an affinity tagged protein, determine the solubility of the protein and purify the His tagged protein using affinity chromatography or ion exchange and an FPLC. The second section of the block will be to cover a powerful bioanalytical instruments mass spectrophotometery. Required Reading all pages are from your textbook, Principles of Biochemistry and Molecular BIology Proteomics - pp392-403 & Wiki link - proteomics ppt Principles of Advanced Chromatography - pp 356 - 378 & 485 - 533 - chromatography ppt - Wiki Chromatography link - Purification User Guide Handout - Handout for Chromatography & FPLC - Recombinant Protein / Affinity Chromatography - Recombinant Protein Expression Paper - Pharmacia Recombinant Purification Book (Not required) Protein Expression (bacterial, yeast, plant, insect and mammalian cell) - Handout above and TBA - Protein Expression (highly detailed handout) - Flow Chart of Protein Expression (great link not required - use handout above) - Yeast Expression FPLC/HPLC - Handout above and TBA - FPLC ppt Mass Spectrometry - pp 405 - 477 & Wiki link - Mass Spec ppt Expression and Advanced Chromatography Experiments Experiment 1 - Small Scale Expression Screen Experiment 2 - Scale-Up Expression Experiment 3 - FPLC Purification of MHG USDA Mass Spec Facillities Field Trip 15 Oct Meet at north SL building door at 2:00 |
Animal and Plant Cell
Culture Drs. Marry & Provost Required Reading - Princ of Biochem & Molec Bio. Chpt 2 - At the Bench Chpt 10 (focus less on the protocols than the background information) - Cell Culture Protocol - Useful Culture Numbers - Cell Culture Lecture ppt (Lecture Notes) - Mammalian Transfection Class Notes - Counting Cells with a Hemocytometer Animal Cell Culture- Animal Cell Culture Experiment Instructions (Optional - not extra credit: no points for grade) Plant Cell Culture Plant Cell Culture Protocol (Optional - not extra credit: no points for grade) Intro to Plant Cell Culture What is a Plant Hormone? Plant Cell Culture Lecture ppt Plant Cell Wall Background (Required Reading) |
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| BCBT 479 Advanced DNA & RNA Methodology Oct 27 - Nov 19; Dr. Chastain Required Reading Notes TBA |
Proposal
Writing Web Links
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